Implementation of High-Throughput Sequencing (HTS) in Aptamer Selection Technology

International Journal of Molecular Sciences

Alexander Kuznetsov¹  Natalia Komarova¹  Daria Barkova¹ 

[1] Scientific-Manufacturing Complex Technological Centre, 1–7 Shokin Square, Zelenograd, 124498 Moscow, Russia;

关键词: aptamer;    SELEX;    high-throughput sequencing;    next-generation sequencing;    random library;    evolution;   

DOI  :  10.3390/ijms21228774

来源: DOAJ

【 摘 要 】

Aptamers are nucleic acid ligands that bind specifically to a target of interest. Aptamers have gained in popularity due to their high potential for different applications in analysis, diagnostics, and therapeutics. The procedure called systematic evolution of ligands by exponential enrichment (SELEX) is used for aptamer isolation from large nucleic acid combinatorial libraries. The huge number of unique sequences implemented in the in vitro evolution in the SELEX process imposes the necessity of performing extensive sequencing of the selected nucleic acid pools. High-throughput sequencing (HTS) meets this demand of SELEX. Analysis of the data obtained from sequencing of the libraries produced during and after aptamer isolation provides an informative basis for precise aptamer identification and for examining the structure and function of nucleic acid ligands. This review discusses the technical aspects and the potential of the integration of HTS with SELEX.

【 授权许可】

Unknown