Characterization of Epigallocatechin-Gallate-Grafted Chitosan Nanoparticles and Evaluation of Their Antibacterial and Antioxidant Potential

Polymers

Abril Z. Graciano-Verdugo¹  María J. Moreno-Vásquez¹  Francisco J. Castillo-Yañez¹  Idania E. Quintero-Reyes²  Judith C. Tanori-Córdova³  Maribel Plascencia-Jatomea⁴  Saúl Sánchez-Valdes⁵ 

[1] Departamento de Ciencias Químico Biológicas, Universidad de Sonora, 83000 Hermosillo, Sonora, Mexico;Departamento de Ciencias de la Salud, Universidad de Sonora, 85040 Cd. Obregón, Sonora, Mexico;Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, 83000 Hermosillo, Sonora, Mexico;Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, 83000 Hermosillo, Sonora, Mexico;Departamento de Procesos de Transformación de Plásticos, Centro de Investigación en Química Aplicada, 25294 Saltillo, Coahuila, Mexico;

关键词: nanoparticles;    modified chitosan;    grafted chitosan;    epigallocatechin gallate;    antibacterial;    antioxidant;   

DOI  :  10.3390/polym13091375

来源: DOAJ

【 摘 要 】

Nanoparticles based on chitosan modified with epigallocatechin gallate (EGCG) were synthetized by nanoprecipitation (EGCG-g-chitosan-P). Chitosan was modified by free-radical-induced grafting, which was verified by Fourier transform infrared (FTIR). Furthermore, the morphology, particle size, polydispersity index, and zeta potential of the nanoparticles were investigated. The grafting degree of EGCG, reactive oxygen species (ROS) production, antibacterial and antioxidant activities of EGCG-g-chitosan-P were evaluated and compared with those of pure EGCG and chitosan nanoparticles (Chitosan-P). FTIR results confirmed the modification of the chitosan with EGCG. The EGCG-g-chitosan-P showed spherical shapes and smoother surfaces than those of Chitosan-P. EGCG content of the grafted chitosan nanoparticles was 330 μg/g. Minimal inhibitory concentration (MIC) of EGCG-g-chitosan-P (15.6 μg/mL) was lower than Chitosan-P (31.2 μg/mL) and EGCG (500 μg/mL) against Pseudomonas fluorescens (p < 0.05). Additionally, EGCG-g-chitosan-P and Chitosan-P presented higher Staphylococcus aureus growth inhibition (100%) than EGCG at the lowest concentration tested. The nanoparticles produced an increase of ROS (p < 0.05) in both bacterial species assayed. Furthermore, EGCG-g-chitosan-P exhibited higher antioxidant activity than that of Chitosan-P (p < 0.05) in 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and ferric-reducing antioxidant power assays. Based on the above results, EGCG-g-chitosan-P shows the potential for food packaging and biomedical applications.

【 授权许可】

Unknown