Enrichment of novel Verrucomicrobia, Bacteroidetes, and Krumholzibacteria in an oxygen‐limited methane‐ and iron‐fed bioreactor inoculated with Bothnian Sea sediments

MicrobiologyOpen

Mike S. M. Jetten¹  Anniek deJong¹  Olivia Rasigraf¹  Paula Dalcin Martins¹  Cornelia U. Welte¹  Niels A. G. M. vanHelmond²  Caroline P. Slomp²  Wytze K. Lenstra² 

[1]Department of Microbiology Radboud University Nijmegen Nijmegen The Netherlands

[2]Netherlands Earth System Science Centre (NESSC) Utrecht The Netherlands

关键词: Bothnian Sea;    coastal sediments;    iron cycling;    low oxygen;    methane oxidation;    methanotrophs;   

DOI  :  10.1002/mbo3.1175

来源: DOAJ

【 摘 要 】

Abstract Microbial methane oxidation is a major biofilter preventing larger emissions of this powerful greenhouse gas from marine coastal areas into the atmosphere. In these zones, various electron acceptors such as sulfate, metal oxides, nitrate, or oxygen can be used. However, the key microbial players and mechanisms of methane oxidation are poorly understood. In this study, we inoculated a bioreactor with methane‐ and iron‐rich sediments from the Bothnian Sea to investigate microbial methane and iron cycling under low oxygen concentrations. Using metagenomics, we investigated shifts in microbial community composition after approximately 2.5 years of bioreactor operation. Marker genes for methane and iron cycling, as well as respiratory and fermentative metabolism, were identified and used to infer putative microbial metabolism. Metagenome‐assembled genomes representing novel Verrucomicrobia, Bacteroidetes, and Krumholzibacteria were recovered and revealed a potential for methane oxidation, organic matter degradation, and iron cycling, respectively. This work brings new hypotheses on the identity and metabolic versatility of microorganisms that may be members of such functional guilds in coastal marine sediments and highlights that microorganisms potentially composing the methane biofilter in these sediments may be more diverse than previously appreciated.

【 授权许可】

Unknown