Carbamazepine Reduces Sharp Wave-Ripple Complexes and Exerts Synapse-Specific Inhibition of Neurotransmission in Ex Vivo Hippocampal Slices

Brain Sciences

Mary Y. Yeh¹  Segewkal H. Heruye¹  Stephanie A. Matthews¹  Kristina A. Simeone¹  Joseph A. Kostansek¹  Kaeli K. Samson¹  Timothy A. Simeone¹ 

[1] Department of Pharmacology & Neuroscience, Creighton University School of Medicine, Omaha, NE 68174, USA;

关键词: cognitive impairment;    epilepsy;    adverse effect;    high frequency oscillations;   

DOI  :  10.3390/brainsci11060787

来源: DOAJ

【 摘 要 】

Higher therapeutic concentrations of the antiseizure medication carbamazepine (CBZ) are associated with cognitive side effects. Hippocampal sharp wave-ripple complexes (SPW-Rs) are proposed to participate in memory consolidation during periods of quiet and slow-wave sleep. SPW-Rs are generated in the CA3 region and are regulated by multiple synaptic inputs. Here, we used a multi-electrode array to determine the effects of CBZ on SPW-Rs and synaptic transmission at multiple hippocampal synapses. Our results demonstrate that CBZ reduced SPW-Rs at therapeutically relevant concentrations (IC₅₀ = 37 μM) and altered the core characteristics of ripples, important for information processing and consolidation. Moreover, CBZ inhibited neurotransmission in a synapse-specific manner. CBZ inhibition was most potent at the medial-perforant-path-to-CA3 and mossy-fiber-to-CA3 synapses (IC₅₀s ~ 30 and 60 μM, respectively) and least potent at medial-perforant-path-to-dentate granule cell synapses (IC₅₀ ~ 120 μM). These results suggest that the synapse-specific CBZ inhibition of neurotransmission reduces SPW-Rs and that the CBZ inhibition of SPW-Rs may underlie the cognitive impairments observed with therapeutic doses of CBZ.

【 授权许可】

Unknown