Longitudinal analysis of humoral immunity against SARS-CoV-2 Spike in convalescent individuals up to 8 months post-symptom onset

Cell Reports Medicine

Jérémie Prévost¹  Chantal Morrisseau¹  Cécile Tremblay²  Guillaume Goyette²  Romain Gasser²  Annemarie Laumaea²  Guillaume Beaudoin-Bussières²  Elsa Brunet-Ratnasingham²  Valérie Martel-Laferrière³  Renée Bazin³  Ralf Duerr³  Halima Medjahed³  Daniel E. Kaufmann³  Gabrielle Gendron-Lepage³  Shang Yu Gong³  Philippe Bégin³  Mehdi Benlarbi³  Nathalie Brassard³  Catherine Bourassa³  Andrés Finzi³  Manon Nayrac³  Jonathan Richard³  Laurie Gokool⁴  Sai Priya Anand⁴ 

[1] Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada;Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada;Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada;Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada;

关键词: coronavirus;    COVID-19;    SARS-CoV-2;    Spike glycoproteins;    RBD;    antibodies;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: With the recent approval of highly effective coronavirus disease 2019 (COVID-19) vaccines, functional and lasting immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently under investigation as antibody levels in plasma were shown to decline during convalescence. Since the absence of antibodies does not equate to absence of immune memory, we evaluate the presence of SARS-CoV-2-specific memory B cells in convalescent individuals. Here, we report a longitudinal assessment of humoral immune responses on 32 donors up to 8 months post-symptom onset. Our observations indicate that anti-Spike and anti-receptor binding domain (RBD) immunoglobulin M (IgM) in plasma decay rapidly, whereas the reduction of IgG is less prominent. Neutralizing activity also declines rapidly when compared to Fc-effector functions. Concomitantly, the frequencies of RBD-specific IgM+ B cells wane significantly when compared to RBD-specific IgG+ B cells, which remain stable. Our results add to the current understanding of immune memory following SARS-CoV-2 infection, which is critical for secondary infection prevention and vaccine efficacy.

【 授权许可】

Unknown