A Microencapsulated Mixture of Eugenol and Garlic Tincture Supplementation Mitigates the Effect of Necrotic Enteritis on Intestinal Integrity and Increases Goblet Cells in Broilers

Microorganisms

Catherine Ionescu¹  Alexandra Blanchard¹  Yadav S. Bajagai²  Shu-Biao Wu³  Sarbast K. Kheravii³  Alip Kumar³  Reza Barekatain⁴ 

[1] ADMi

[2] Pancosma SA, A-One Business Center, La Piece 3, CH-1180 Rolle, Switzerland;Institute for Future Farming Systems, Central Queensland University, Rockhampton, QLD 4702, Australia;School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia;South Australian Research and Development Institute, Roseworthy Campus, University of Adelaide, Roseworthy, SA 5371, Australia;

关键词: plant extract;    intestinal health;    alternative to antimicrobials;    clinical necrotic enteritis;    broiler chicken;   

DOI  :  10.3390/microorganisms9071451

来源: DOAJ

【 摘 要 】

This study was conducted to examine the effects of a plant extract mixture, a microencapsulated product composed of eugenol and garlic tincture (PE), on intestinal health in broilers under necrotic enteritis (NE) challenge. A total of 960 d-old mixed-sex Cobb 500 chicks were randomly distributed to 48-floor pens housing 20 birds per pen. Six treatments were applied: UC, unchallenged control; CC, challenged control; PE, challenged group plus PE; AM, challenged group plus antimicrobial (AM); FAP, challenged group plus a full dose of AM with PE; HAP, challenged group plus a half dose of AM with PE in starter, grower and finisher phases. Birds in the challenged groups were inoculated with Eimeria spp. on d 9 and Clostridiumperfringens on d 14. On d 16, the CC group had increased serum fluorescein isothiocyanate dextran (FITC-d), reduced villus surface area, goblet cell number, upregulated CLDN1, JAM2 genes and reduced microbial diversity compared to the UC group (p < 0.05). Birds fed PE had reduced FITC-d, increased goblet cell number and Bifidobacterium compared to the CC group (p < 0.05). Birds fed PE had reduced CLDN5 expression in male birds, and Bacteroides spp. in female birds than CC group (p < 0.05). These findings suggest that PE supplementation mitigates the effect of NE by improving the intestinal health of birds.

【 授权许可】

Unknown