A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites

Frontiers in Immunology

Ping Liang¹  Bing Xie¹  Chengbo Long²  Qiumin Lu²  Yanling Deng²  Ren Lai³  Jiayao Li⁵  Chuanbin Shen⁶  Feilong Wu⁶  Yongzhi Yu⁷ 

[1] Genetic Analysis of Model Animals (Primate Facility), Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China;;Local Joint Engineering Center of Natural bioactive Peptides, Kunming Institute of Zoology-The Chinese University of Hong Kong (KIZ-CUHK) Joint Laboratory of Bioresources and Molecular Research in Common Diseases, National Resource Center for Non-Human Primates, Kunming Primate Research Center, and National Research Facility for Phenotypic &Department of Laboratory Medicine, Li Ka Shing Knowledge Institute (LKSKI)-Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, University of Toronto, Toronto, ON, Canada;Institute of Biology Leiden, Leiden University, Leiden, Netherlands;;Key Laboratory of Animal Models and Human Disease Mechanisms, Key Laboratory of Bioactive Peptides of Yunnan Province, Engineering Laboratory of Bioactive Peptides, The National &Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, China;Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, China;

关键词: snakebite envenoming;    venom;    antigen;    immunogenicity;    diagnosis;   

DOI  :  10.3389/fimmu.2021.775678

来源: DOAJ

【 摘 要 】

As said by former United Nations Secretary-General Kofi Annan, “Snakebite is the most important tropical disease you’ve never heard of.” Listed as a priority neglected tropical disease by the World Health Organization, snakebite envenoming (SBE) kills in excess of 125,000 people per year. However, due to the complexity and overlap of snake venom compositions, few reliable venom diagnostic methods for genus-/species-specific identification, which is crucial for successful SBE therapy, are available. Here, we develop a strategy to select and prepare genus-specific snake venom antibodies, which allows rapid and efficient clinical diagnosis of snakebite. Multi-omics approaches are used to choose candidate antigens from snake venoms and identify genus-specific antigenic epitope peptide fragments (GSAEPs) with ideal immunogenicity, specificity, and spatial accessibility. Double-antibody sandwich ELISA kit was established by matching a polyclonal antibody against a natural antigen and a monoclonal antibody that was prepared by natural protein as antigen and can specifically target the GSAEPs. The kit shows the ability to accurately identify venoms from similar genera of Trimeresurus and Protobothrops with a detection limit of 6.25 ng/ml on the snake venoms and a little cross-reaction, thus proving high feasibility and applicability.

【 授权许可】

Unknown