MiRNA‐199a‐5p targets WNT2 to regulate depression through the CREB/BDNF signaling in hippocampal neuron

Brain and Behavior

Hua Shao¹  Jianli Yang¹  Qing Fang¹  Dalu Yang¹  Zheng Liu²  Lizhi Gao³  Junfang Sun³ 

[1] Department of Clinical Psychology Tianjin Medical University General Hospital Tianjin China;Department of Graduate School Tianjin Medical University Tianjin China;Department of department of neurology The Second Affiliated Hospital of Baotou Medical College Baotou China;

关键词: CREB/BDNF;    depression;    miR‐199a‐5p;    WNT2;   

DOI  :  10.1002/brb3.2107

来源: DOAJ

【 摘 要 】

Abstract Introduction This study mainly investigated the role of miR‐199a‐5p in depression. Methods qRT‐PCR and western blotting were employed to detect the expressions of miR‐199a‐5p, CREB and BDNF. Sucrose preference test, forced swimming test, and tail suspension test were performed to evaluate depression‐related symptoms. MTT assays and flow cytometry were used to examine the cell reproduction and apoptotic cells of hippocampal neuron. Results The data demonstrated that the expression levels of miR‐199a‐5p in the cerebrospinal fluids and serums of depression patient and the hippocampus of chronic unpredictable mild stress (CUMS) mouse were significantly increased. However, the expressions of WNT2, p‐CREB, and BDNF were inhibited. In addition, miR‐199a‐5p‐inhibitor enhanced sucrose preferences of CUMS mouse and decreased immobile time in sucrose preference test and forced swimming test. Knockdown of WNT2 attenuated the effects of miR‐199a‐5p‐inhibitor on cell reproduction and apoptotic cells of hippocampal neuron and the expression of WNT2, p‐CREB, and BDNF. Conclusion MiR‐199a‐5p can target WNT2 to enhance the development of depression through regulation of the CREB/BDNF signaling. Trial registration: JNU‐Hos‐49284.

【 授权许可】

Unknown