Technique Evolutions for Microorganism Detection in Complex Samples: A Review

Applied Sciences

Fany Reffuveille¹  Sophie C. Gangloff¹  Pierre Prada²  Benjamin Brunel² 

[1] Université de Reims Champagne-Ardenne, EA 4691-Biomatériaux et Inflammation en Site Osseux (BIOS), UFR de Pharmacie, SFR Cap Santé, 51 rue Cognacq-Jay, 51096 Reims, France;Université de Reims Champagne-Ardenne, EA7506-BioSpectroscopie Translationnelle (BioSpecT), UFR de Pharmacie, 51 rue Cognacq-Jay, 51097 Reims, France;

关键词: pathogen detection;    biosensors;    DNAzyme;    AMP;    aptamer;    paper sensors;   

DOI  :  10.3390/app12125892

来源: DOAJ

【 摘 要 】

Rapid detection of microorganisms is a major challenge in the medical and industrial sectors. In a pharmaceutical laboratory, contamination of medical products may lead to severe health risks for patients, such as sepsis. In the specific case of advanced therapy medicinal products, contamination must be detected as early as possible to avoid late production stop and unnecessary costs. Unfortunately, the conventional methods used to detect microorganisms are based on time-consuming and labor-intensive approaches. Therefore, it is important to find new tools to detect microorganisms in a shorter time frame. This review sums up the current methods and represents the evolution in techniques for microorganism detection. First, there is a focus on promising ligands, such as aptamers and antimicrobial peptides, cheaper to produce and with a broader spectrum of detection. Then, we describe methods achieving low limits of detection, thanks to Raman spectroscopy or precise handling of samples through microfluids devices. The last part is dedicated to techniques in real-time, such as surface plasmon resonance, preventing the risk of contamination. Detection of pathogens in complex biological fluids remains a scientific challenge, and this review points toward important areas for future research.

【 授权许可】

Unknown