Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients

Frontiers in Cellular and Infection Microbiology

Sandra Morales¹  Bradford A. Woodworth²  Marjolein E. Cornet³  Wytske J. Fokkens³  Stephanie A. Fong⁴  Sarah Vreugde⁴  Peter-John Wormald⁴  Alkis J. Psaltis⁴  Amanda Drilling⁴ 

[1] AmpliPhi AustraliaBrookvale, NSW, Australia;Department of Otolaryngology-Head and Neck Surgery, University of Alabama at BirminghamBirmingham, AL, United States;Department of Otorhinolaryngology, Academic Medical Center, University of AmsterdamAmsterdam, Netherlands;Department of Surgery—Otolaryngology, Head and Neck Surgery, The Queen Elizabeth HospitalWoodville South, SA, Australia;

关键词: bacteriophage;    Pseudomonas aeruginosa;    chronic rhinosinusitis;    cystic fibrosis;    biofilm;    multidrug resistant;   

DOI  :  10.3389/fcimb.2017.00418

来源: DOAJ

【 摘 要 】

Introduction:Pseudomonas aeruginosa infections are prevalent amongst chronic rhinosinusitis (CRS) sufferers. Many P. aeruginosa strains form biofilms, leading to treatment failure. Lytic bacteriophages (phages) are viruses that infect, replicate within, and lyse bacteria, causing bacterial death.Aim: To assess the activity of a phage cocktail in eradicating biofilms of ex vivo P.aeruginosa isolates from CRS patients.Methods: P. aeruginosa isolates from CRS patients with and without cystic fibrosis (CF) across three continents were multi-locus sequence typed and tested for antibiotic resistance. Biofilms grown in vitro were treated with a cocktail of four phages (CT-PA). Biofilm biomass was measured after 24 and 48 h, using a crystal violet assay. Phage titrations were performed to confirm replication of the phages. A linear mixed effects model was applied to assess the effects of treatment, time, CF status, and multidrug resistance on the biomass of the biofilm.Results: The isolates included 44 strain types. CT-PA treatment significantly reduced biofilm biomass at both 24 and 48 h post-treatment (p < 0.0001), regardless of CF status or antibiotic resistance. Biomass was decreased by a median of 76% at 48 h. Decrease in biofilm was accompanied by a rise in phage titres for all except one strain.Conclusion: A single dose of phages is able to significantly reduce biofilms formed in vitro by a range of P.aeruginosa isolates from CRS patients. This represents an exciting potential and novel targeted treatment for P. aeruginosa biofilm infections and multidrug resistant bacteria.

【 授权许可】

Unknown