Antifungal and Antioxidant Properties of Chitosan Polymers Obtained from Nontraditional Polybius henslowii Sources

Marine Drugs

LuísF.V. Pinto¹  Paulo Marques Nunes²  Rui Pedrosa²  Francisco Avelelas²  SérgioMiguel Leandro²  André Horta³  Sónia Cotrim Marques³ 

[1] BioCeramed, S.A., Rua José Gomes Ferreira nº 1 - Armazém D 2660-360 São Julião do Tojal, Portugal;MARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, Portugal;MARE—Marine and Environmental Sciences Centre, Instituto Politécnico de Leiria, 2520-641 Peniche, Portugal;

关键词: Polybius henslowii;    marine resources;    chitosan;    chitooligosaccharides;    antifungal activity;    antioxidant activity;   

DOI  :  10.3390/md17040239

来源: DOAJ

【 摘 要 】

Chitin was extracted from Polybius henslowii, a swimming crab, captured in large quantities throughout the Portuguese coast by purse seine vessels as bycatch. After standard chitin extraction procedures, water-soluble chitosan products were obtained via two different methods: (1) N-acetylation with the addition of acetic anhydride and (2) a reaction with hydrogen peroxide. The chemical structure and molecular weight of chitosan derivatives, water-soluble chitosan (WSC) and chitooligosaccharides (COS), were confirmed by Fourier Transform Infrared Spectroscopy (FT-IR) and gel permeation chromatography (GPC). Antioxidant and metal chelation activities were evaluated, and the growth inhibition capacity was tested on four phytopatogens. The chitooligosaccharides from pereopods (pCOS) and shell body parts (sCOS) inhibited all fungal species tested, particularly Cryphonectria parasitica with 84.7% and 85.5%, respectively. Both radical scavenging and antifungal activities proved to be dose-dependent. Chitooligosaccharides with a low molecular weight (2.7, 7.4, and 10.4 Kg·mol⁻¹) showed the highest activity among all properties tested. These results suggested that chitosan derivatives from P. henslowii raw material could potentially be used against phytopathogens or as ingredient in cosmetics and other products related to oxidative stress.

【 授权许可】

Unknown