Novel Aflatoxin-Degrading Enzyme from Bacillus shackletonii L7

Toxins

Fuguo Xing¹  Liang Xu¹  Lancine Sangare¹  Yueju Zhao¹  Yan Wang¹  Mohamed Farah Eisa Ahmed¹  Jonathan Nimal Selvaraj¹  Yang Liu¹  Hongping Yang² 

[1] Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, 1 Nongda South Road, Xibeiwang Town, Haidian District, Beijing 100193, China;Shenyang Institute of Engineering, No.18 Puchang Road, Shenbei New District, Shenyang 110136, China;

关键词: aflatoxin B1;    aflatoxin-degrading enzyme;    biodegradation;    Bacillus shackletonii;    purification;   

DOI  :  10.3390/toxins9010036

来源: DOAJ

【 摘 要 】

Food and feed contamination by aflatoxin (AF)B1 has adverse economic and health consequences. AFB1 degradation by microorganisms or microbial enzymes provides a promising preventive measure. To this end, the present study tested 43 bacterial isolates collected from maize, rice, and soil samples for AFB1-reducing activity. The higher activity was detected in isolate L7, which was identified as Bacillus shackletonii. L7 reduced AFB1, AFB2, and AFM1 levels by 92.1%, 84.1%, and 90.4%, respectively, after 72 h at 37 °C. The L7 culture supernatant degraded more AFB1 than viable cells and cell extracts; and the degradation activity was reduced from 77.9% to 15.3% in the presence of proteinase K and sodium dodecyl sulphate. A thermostable enzyme purified from the boiled supernatant was designated as Bacillus aflatoxin-degrading enzyme (BADE). An overall 9.55-fold purification of BADE with a recovery of 39.92% and an activity of 3.85 × 103 U·mg−1 was obtained using chromatography on DEAE-Sepharose. BADE had an estimated molecular mass of 22 kDa and exhibited the highest activity at 70 °C and pH 8.0, which was enhanced by Cu2+ and inhibited by Zn2+, Mn2+, Mg2+, and Li+. BADE is the major protein involved in AFB1 detoxification. This is the first report of a BADE isolated from B. shackletonii, which has potential applications in the detoxification of aflatoxins during food and feed processing.

【 授权许可】

Unknown