Investigation of Antibody Levels During Three Doses of Sinopharm/BBIBP Vaccine Inoculation

Frontiers in Immunology

Siping Li¹  Shuangshuang Huang²  Yifeng Zeng²  Runpei Lin²  Zhiman Liang²  Yijun Deng²  Shiyun Li²  Jing Ma²  Yuanyi Cheng²  Huimin Huang²  Xuefeng Niu²  Yanting Fang²  Peiyan Zheng²  Baoqing Sun²  Mingshan Xue²  Zhangkai J. Cheng²  Qian Wang²  Huan Liang² 

[1] Clinical Laboratory, Dongguan Eighth People’s Hospital, Dongguan, China;Department of Allergy and Clinical Immunology, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China;

关键词: coronavirus disease 2019;    vaccine;    antibody persistence;    booster;    chemiluminescent immunoassay;   

DOI  :  10.3389/fimmu.2022.913732

来源: DOAJ

【 摘 要 】

Levels of neutralizing antibodies (NAb) after vaccine against coronavirus disease 2019 (COVID-19) can be detected using a variety of methods. A critical challenge is how to apply simple and accurate methods to assess vaccine effect. In a population inoculated with three doses of the inactivated Sinopharm/BBIBP vaccine, we assessed the performance of chemiluminescent immunoassay (CLIA) in its implementation to detect severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) specific antibodies, as well as the antibody kinetics of healthcare workers throughout the course of vaccination. The antibody levels of NAb, the receptor-binding-domain (RBD) antibodies and IgG peaked one month after the second and remained at a relatively high level for over three months after the booster injection, while IgM and IgA levels remained consistently low throughout the course of vaccination. The production of high-level neutralizing antibodies is more likely when the inoculation interval between the first two doses is within the range of one to two months, and that between the first and booster dose is within 230 days. CLIA showed excellent consistency and correlation between NAb, RBD, and IgG antibodies with the cytopathic effect (CPE) conventional virus neutralization test (VNT). Receiver operating characteristic (ROC) analysis revealed that the optimal cut-off levels of NAb, RBD and IgG were 61.77 AU/ml, 37.86 AU/ml and 4.64 AU/ml, with sensitivity of 0.833, 0.796 and 0.944, and specificity of 0.768, 0.750 and 0.625, respectively, which can be utilized as reliable indicators of COVID-19 vaccination immunity detection.

【 授权许可】

Unknown