Effect of Progesterone on Expression of MMP7 and MMP13 in Lungs of Female Mice

Iranian Journal of Allergy, Asthma and Immunology

Jalil Kuhpayezadeh¹  Fateme Vafashoar²  Pendar Safari²  Paria Jorbozedar²  Mohamad Ali Assarehzadegan²  Nazanin Mojtabavi²  Elahe Izadi³  Hadi Poormoghim⁴ 

[1] Community Medicine, Preventive Medicine and Public Health Research Center, Iran University of Medical Sciences, Tehran, Iran;Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;International Campus, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;Scleroderma Study Group, Firuzgar Hospital, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;

关键词: Collagenase;    Extracellular matrix;    Lung diseases;    Matrilysin;    Matrix metalloproteinases;    Progesterone;   

DOI  :  10.18502/ijaai.v17i5.306

来源: DOAJ

【 摘 要 】

Gender medicine is a new era of science which focuses on the impact of sex hormones and gender on normal physiology, pathobiology and clinical features of diseases. In this study we investigated the impact of pregnancy doses of progesterone hormone on the expression of a couple of matrix metalloproteinase (MMPs), which are known to be involved in tissue remodeling of lungs in health and disease, namely MMP7 and 13. Pregnancy maintenance dose of progesterone was administered to female BALB/c mice for 21 and 28 days, the control group received PBS for the same days. After removal of the lungs and RNA extraction, quantitative real-time PCR was done using specific primers for MMP7 and MMP13. We found that progesterone can slightly (not significantly) decrease the expression of MMP13 but had no effect on MMP7. Our results shows that progesterone has minimal effect on the expression of matrix metalloproteinase7 and matrix metalloproteinase 13, but it may still have an effect on corresponding tissue inhibitor of matrix metalloproteinases (TIMPs) or other components of the Extracellular matrix  which remains to be elucidated. Also, the effect of progesterone on these MMPs can be further studied in a fibrosis model.

【 授权许可】

Unknown