Prevalence of bee viruses in Apis cerana cerana populations from different locations in the Fujian Province of China

MicrobiologyOpen

Aqai Kalan Hassanyar¹  Muhammad Fahad Raza¹  Zhiguo Li¹  Muhammad Rizwan¹  Shaokang Huang¹  Songkun Su¹  Mubasher Hussain²  Muhammad Qasim²  Shahid Mehmood³ 

[1] College of Bee Science Fujian Agriculture and Forestry University Fuzhou China;College of Plant Protection Fujian Agriculture and Forestry University Fuzhou China;Key Laboratory of Tropical Forest Ecology Chemical Ecology Group Xishuangbanna Tropical Botanical GardenChinese Academy of Sciences Kunming China;

关键词: Apis cerana cerana;    Fujian;    honeybee;    prevalence;    RT‐PCR;    viruses;   

DOI  :  10.1002/mbo3.830

来源: DOAJ

【 摘 要 】

Abstract Prevalence of honeybee viral diseases has recently been causing major problems in the beekeeping industry, causing economic losses worldwide. Honeybees are susceptible to a variety of diseases and various pathogens. Among these pathogens, prevalence viruses, along with other factors, are seriously threatening the health of bee species. In the present study, samples were collected from 80 Apis cerana cerana (A. c. cerana) colonies from three different locations, Cangshan, Fuan, and Yongtai, in the Fujian Province of China. All samples were screened using the reverse transcription polymerase chain reaction (RT‐PCR) method for detection of seven honeybee viruses, namely, Chinese sacbrood virus (CSBV), deformed wing virus (DWV), Israeli acute paralysis virus (IAPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV), and Kashmir bee virus (KBV). Our results showed that CSBV was the most prevalent as it was detected in (90%), of the samples, DWV was detected in (81.25%), and IAPV was detected in (26.25%). In contrast, insignificant prevalence results were obtained from all apiaries for BQCV, CBPV, APBV, and KBV, which were not detected in any sample. Here, we are providing the first report on the molecular detection of honeybee viruses, especially the prevalence of IAPV, from different regions in the Fujian Province of China with a high prevalence of bee viruses, on A. c. cerana, and there is great concern for the presence of honeybee viruses in the population of the native honeybee (A. c. cerana) in China.

【 授权许可】

Unknown