Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant

BMC Plant Biology

Rajeev K. Varshney¹  Yue Liu²  Yong Lei²  Xia Wan²  Boshou Liao²  Liying Yan²  Liyun Wan²  Manish K. Pandey³  Jiahai Fang³ 

[1] Center of Excellence in Genomics, International Crops Research Institute for the Semi-Arid Tropics;Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences;Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University;

关键词: Peanut (Arachis hypogaea L.);    Seed coat;    Hormone;    Flavonoid;    Transcriptional regulation;    Metabolome;   

DOI  :  10.1186/s12870-020-02383-7

来源: DOAJ

【 摘 要 】

Abstract Background Coat color determines both appearance and nutrient quality of peanut. White seed coat in peanut can enhance the processing efficiency and quality of peanut oil. An integrative analysis of transcriptomes, metabolomes and histocytology was performed on wsc mutant and its wild type to investigate the regulatory mechanisms underlying color pigmentation. Result Metabolomes revealed flavonoids were redirected in wsc, while multi-omics analyses of wsc mutant seeds and testae uncovered WSC influenced the flavonoids biosynthesis in testa as well as suberin formation, glycolysis, the TCA cycle and amino acid metabolism. The mutation also enhanced plant hormones synthesis and signaling. Further, co-expression analysis showed that FLS genes co-expressed with MBW complex member genes. Combining tissue expression patterns, genetic analyses, and the annotation of common DEGs for these three stages revealed that three testa specific expressed candidate genes, Araip.M7RY3, Aradu.R8PMF and Araip.MHR6K were likely responsible for the white testa phenotype. WSC might be regulated expression competition between FLS and DFR by controlling hormone synthesis and signaling as well as the MBW complex. Conclusions The results of this study therefore provide both candidate genes and novel approaches that can be applied to improve peanut with desirable seed coat color and flavonoid quality.

【 授权许可】

Unknown