Substitution Mapping of the Major Quantitative Trait Loci Controlling Stigma Exsertion Rate from Oryza glumaepatula

Rice

Guifu Liu¹  Zifeng Yang¹  Yuerong Ni¹  Xin Luan¹  Haitao Zhu¹  Mingmin Zheng¹  Xuelin Fu¹  Xiaohui Li¹  Guiquan Zhang¹  Tuo Zou¹  Shaokui Wang¹  Weifeng Yang¹  Quanya Tan¹  Ruizhen Zeng¹ 

[1] Guangdong Provincial Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University;

关键词: Stigma exsertion;    Outcrossing;    Quantitative trait locus;    Single-segment substitution line;    Wild species;    Rice;   

DOI  :  10.1186/s12284-020-00397-1

来源: DOAJ

【 摘 要 】

Abstract Background Stigma exsertion rate (SER) is a key determinant for the outcrossing ability of male sterility lines (MSLs) in hybrid rice seed production. In the process of domestication, the outcrossing ability of cultivated rice varieties decreased, while that of wild Oryza species kept strong. Here, we detected the quantitative trait loci (QTLs) controlling SER using a set of single-segment substitution lines (SSSLs) derived from O. glumaepatula, a wild Oryza species. Results Seven QTLs for SER were located on 5 chromosomes. qSER-1a and qSER-1b were located on chromosome 1. qSER-3a and qSER-3b were mapped on chromosome 3, and qSER-3b was further located at an estimated interval of 898.8 kb by secondary substitution mapping. qSER-5, qSER-9 and qSER-10 were identified on chromosomes 5, 9 and 10, respectively, and qSER-9 was delimited to an estimated region of 551.9 kb by secondary substitution mapping. The additive effects of the 7 QTLs ranged from 10.6% to 14.8%, which were higher than those of most loci for SER reported previously. Conclusions qSER-1a and qSER-1b are novel loci for SER on chromosome 1. All of the 7 QTLs have major effects on SER. The major QTLs of SER will help to develop MSLs with strong outcrossing ability.

【 授权许可】

Unknown