Evaluation of Hydrogen Cyanide in the Blood of Fire Victims Based on the Kinetics of the Reaction with Ninhydrin

Applied Sciences

Gabi Drochioiu¹  Daniel Tabian²  Sebastian Ionut Toma²  Mihaela Badea²  Diana Bulgaru Iliescu³  Madalina Maria Diac³ 

[1] Faculty of Chemistry, “Alexandru Ioan Cuza” University of Iasi, 11 Carol I, 700605 Iasi, Romania;Faculty of Medicine, Transilvania University of Brasov, 56 Nicolae Balcescu Street, 500019 Brasov, Romania;Faculty of Medicine, “Grigore T. Popa” University of Medicine and Pharmacy Iasi, 16 Universitatii Street, 700115 Iasi, Romania;

关键词: cyanide determination;    forensic science;    fire victims;    blood samples;    kinetics;    ninhydrin;   

DOI  :  10.3390/app12052329

来源: DOAJ

【 摘 要 】

An original kinetic spectrophotometric procedure was developed for the determination of hydrogen cyanide (HCN) in the whole blood of fire victims. Cyanide poisoning by smoke inhalation is common in forensic medicine, but the blood HCN of fire victims has not been studied in detail so far. In this research project, we developed a simple, fast, sensitive, and selective quantification method for both free and metabolized HCN based on the kinetics of cyanide reaction with ninhydrin. The method was linear in range, from 0.26 to 2.6 μg mL⁻¹, with a coefficient of determination of r = 0.994. A high molar absorptivity of 4.95 × 10⁵ L mol⁻¹ cm⁻¹ was calculated under the reaction conditions. The limit of quantification was 0.052 μg mL⁻¹; the detection limit was 0.012 μg mL⁻¹ and the standard error was ±2.7%. This micro method proved to be accurate, sensitive, and selective and has been successfully applied to the analysis of blood samples, allowing rapid monitoring of blood cyanide in several fire victims.

【 授权许可】

Unknown