GC-MS Metabolomics and Antifungal Characteristics of Autochthonous Lactobacillus Strains

Dairy

Pierluigi Caboni¹  Antonio Murgia¹  Paola Scano¹  M. Barbara Pisano²  Sofia Cosentino² 

[1] Department of Life and Environmental Sciences, University of Cagliari, SS 554 km 4.5, 09042 Monserrato, Italy;Department of Medical Sciences and Public Health, University of Cagliari, SS 554 km 4.5, 09042 Monserrato, Italy;

关键词: Lactobacillus;    antifungal activity;    fresh cheese;    biopreservation;    GC-MS;    metabolomics;   

DOI  :  10.3390/dairy2030026

来源: DOAJ

【 摘 要 】

Lactobacillus strains with the potential of protecting fresh dairy products from spoilage were studied. Metabolism and antifungal activity of different L. plantarum, L. brevis, and L. sakei strains, isolated from Sardinian dairy and meat products, were assessed. The metabolite fingerprint of each strain was obtained by GC-MS and data submitted to multivariate statistical analysis. The discriminant analysis correctly classified samples to the Lactobacillus species and indicated that, with respect to the other species, L. plantarum had higher levels of organic acids, while L. brevis and L. sakei showed higher levels of sugars than L. plantarum. Partial Least Square (PLS) regression correlated the GC-MS metabolites to the antifungal activity (p < 0.05) of Lactobacillus strains and indicated that organic acids and oleamide are positively related with this ability. Some of the metabolites identified in this study have been reported to possess health promoting proprieties. These overall results suggest that the GC-MS-based metabolomic approach is a useful tool for the characterization of Lactobacillus strains as biopreservatives.

【 授权许可】

Unknown