Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae Pyropia Yezoensis and Its Biological Activities

Molecules

Youn-Hee Choi¹  Selvakumari Ulagesan¹  Taek-Jeong Nam² 

[1] Department of Marine Bio-Materials & Aquaculture, Pukyong National University, Nam-gu, Busan 48513, Korea;Institute of Fisheries Sciences, Pukyong National University, Gijang-gun, Busan 46041, Korea;

关键词: Pyropia yezoensis;    marine algae;    phycoerythrin;    antioxidant activity;    cytotoxicity;    anticancer activity;   

DOI  :  10.3390/molecules26216479

来源: DOAJ

【 摘 要 】

Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that is widely used as a fluorescent probe or as a colorant in the food and cosmetic industries. In this study, phycoerythrin was extracted from the red algae Pyropia yezoensis and purified by ammonium sulfate precipitation and various chromatography methods. The purified phycoerythrin was analyzed by UV-visible and fluorescence spectroscopy. The isolated pigment had the typical spectrum of R-phycoerythrin, with a trimmer state with absorbance maxima at 497, 536, and 565 nm. It was further purified and identified by LC-MS/MS and Mascot search. It showed a 100% sequence similarity with the R-phycoerythrin alpha subunit of Pyropia yezoensis. The molecular mass was 17.97 kDa. The antioxidant activity of the purified R-phycoerythrin alpha subunit was analyzed. It showed significant antioxidant activity in ABTS and FRAP assays and had significant cytotoxicity against HepG2 cells.

【 授权许可】

Unknown