Hsa_circ_0005379 regulates malignant behavior of oral squamous cell carcinoma through the EGFR pathway

BMC Cancer

Minghua Li¹  Feng Wang²  Shuai Sun²  Hongyu Yang²  Wen Su²  Yuehong Shen²  Yufan Wang² 

[1] Central laboratory, Peking University Shenzhen Hospital;Department of Oral and Maxillofacial Surgery, Peking University Shenzhen Hospital;

关键词: Oral squamous cell carcinoma;    Circular RNAs;    EGFR pathway;    Cetuximab;    Epithelial–mesenchymal transition;   

DOI  :  10.1186/s12885-019-5593-5

来源: DOAJ

【 摘 要 】

Abstract Background Oral squamous cell carcinoma (OSCC) is an oral and maxillofacial malignancy with a high incidence worldwide. Accumulating evidence indicates that circular RNAs (circRNAs) play a vital role in modulating tumor development. However, the mechanism of circRNA action in human OSCC remains largely unknown. Methods By using high-throughput transcriptome sequencing technology, we conducted a comprehensive study of circRNAs in human OSCC. The effect of circRNA hsa_circ_0005379 on OSCC tissues and cell lines was monitored by qRT-PCR, Transwell assay, flow cytometry, and western blot analysis. Xenograft mouse models were used to assess tumor growth and animal survival. Results We found that circRNA hsa_circ_0005379 expression is significantly lower in OSCC tissue compared to paired non-cancerous matched tissue and is associated with tumor size and differentiation. Overexpression of hsa_circ_0005379 effectively inhibits migration, invasion, and proliferation of OSCC cells in vitro and suppresses OSCC growth in nude mice in vivo. Mechanistic studies revealed that hsa_circ_0005379 may be involved in the regulation of the epidermal growth factor receptor (EGFR) pathway. Furthermore, we found that high expression of hsa_circ_0005379 could significantly enhance the sensitivity of OSCC to the cetuximab drug. Conclusions Our findings provide evidence that hsa_circ_0005379 regulates OSCC malignancy and may be a new therapeutic target for OSCC treatment.

【 授权许可】

Unknown