Identification of circulating microRNA profiles associated with pulmonary function and radiologic features in survivors of SARS-CoV-2-induced ARDS

Emerging Microbes and Infections

Dario Garcia-Gasulla¹  Jesús F. Bermejo-Martin²  Ricard Ferrer²  Rosario Menéndez²  Adrián Ceccato²  Ana Motos²  Antoni Torres²  Jordi Riera²  Laia Fernández-Barat²  Oscar Peñuelas²  Jesús Caballero³  Estefania Nova-Lamperti⁴  Gonzalo Labarca⁴  Paola Carmona⁵  Jessica González⁵  David de Gonzalo-Calvo⁵  Sally Santisteve⁵  Manel Pérez-Pons⁵  Iván D. Benítez⁵  María C. García-Hidalgo⁵  Anna Moncusí-Moix⁵  Ferran Barbé⁵  Fátima Rodríguez-Jara⁵  Clara Gort-Paniello⁵  Marta Molinero⁵  Thalia Belmonte⁵  Gerard Torres⁵ 

[1] Barcelona Supercomputing Center (BSC), Barcelona, Spain;CIBER of Respiratory Diseases (CIBERES), Institute of Health Carlos III, Madrid, Spain;Grup de Recerca Medicina Intensiva, Intensive Care Department Hospital Universitari Arnau de Vilanova, IRBLleida, Lleida, Spain;Molecular and Translational Immunology Laboratory, Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, Universidad de Concepcion, Concepcion, Chile;Translational Research in Respiratory Medicine, University Hospital Arnau de Vilanova and Santa Maria, IRBLleida, Lleida, Spain;

关键词: Acute respiratory distress syndrome;    COVID-19;    lung function;    microRNA;    sequelae;    total severity score;   

DOI  :  10.1080/22221751.2022.2081615

来源: DOAJ

【 摘 要 】

There is a limited understanding of the pathophysiology of postacute pulmonary sequelae in severe COVID-19. The aim of current study was to define the circulating microRNA (miRNA) profiles associated with pulmonary function and radiologic features in survivors of SARS-CoV-2-induced ARDS. The study included patients who developed ARDS secondary to SARS-CoV-2 infection (n = 167) and a group of infected patients who did not develop ARDS (n = 33). Patients were evaluated 3 months after hospital discharge. The follow-up included a complete pulmonary evaluation and chest computed tomography. Plasma miRNA profiling was performed using RT-qPCR. Random forest was used to construct miRNA signatures associated with lung diffusing capacity for carbon monoxide (DLCO) and total severity score (TSS). Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses were conducted. DLCO < 80% predicted was observed in 81.8% of the patients. TSS showed a median [P25;P75] of 5 [2;8]. The miRNA model associated with DLCO comprised miR-17-5p, miR-27a-3p, miR-126-3p, miR-146a-5p and miR-495-3p. Concerning radiologic features, a miRNA signature composed by miR-9-5p, miR-21-5p, miR-24-3p and miR-221-3p correlated with TSS values. These associations were not observed in the non-ARDS group. KEGG pathway and GO enrichment analyses provided evidence of molecular mechanisms related not only to profibrotic or anti-inflammatory states but also to cell death, immune response, hypoxia, vascularization, coagulation and viral infection. In conclusion, diffusing capacity and radiological features in survivors from SARS-CoV-2-induced ARDS are associated with specific miRNA profiles. These findings provide novel insights into the possible molecular pathways underlying the pathogenesis of pulmonary sequelae.Trial registration: ClinicalTrials.gov identifier: NCT04457505..Trial registration: ISRCTN.org identifier: ISRCTN16865246..

【 授权许可】

Unknown