Soft-Lithography of Polyacrylamide Hydrogels Using Microstructured Templates: Towards Controlled Cell Populations on Biointerfaces

Materials

GustavoR. Plaza¹  Markus Guttmann²  Florian Rupp²  Marc Schneider²  Markus Wissmann²  Stefan Hengsbach²  Klaus Bade²  Matthias Worgull²  Andrés Díaz Lantada³  Noelia Mazarío Picazo³ 

[1] Centre for Biomedical Technology, Universidad Politécnica de Madrid, Parque Científico y Tecnológico de la UPM, Crta. M40, km. 38, 28223 Pozuelo de Alarcón, Madrid, Spain;Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz Platz 1, 76344 Eggenstein-Leopoldshafen, Germany;Product Development Laboratory, Mechanical Engineering Department, Universidad Politécnica de Madrid, c/José Gutiérrez Abascal 2, 28006 Madrid, Spain;

关键词: polymer microfabrication;    polyacrylamide hydrogels;    hot-embossing;    soft-lithography;    biointerfaces;    surface patterning;   

DOI  :  10.3390/ma13071586

来源: DOAJ

【 摘 要 】

Polyacrylamide hydrogels are interesting materials for studying cells and cell–material interactions, thanks to the possibility of precisely adjusting their stiffness, shear modulus and porosity during synthesis, and to the feasibility of processing and manufacturing them towards structures and devices with controlled morphology and topography. In this study a novel approach, related to the processing of polyacrylamide hydrogels using soft-lithography and employing microstructured templates, is presented. The main novelty relies on the design and manufacturing processes used for achieving the microstructured templates, which are transferred by soft-lithography, with remarkable level of detail, to the polyacrylamide hydrogels. The conceived process is demonstrated by patterning polyacrylamide substrates with a set of vascular-like and parenchymal-like textures, for controlling cell populations. Final culture of amoeboid cells, whose dynamics is affected by the polyacrylamide patterns, provides a preliminary validation of the described strategy and helps to discuss its potentials.

【 授权许可】

Unknown