Formation of Giant Lipid Vesicles in the Presence of Nonelectrolytes—Glucose, Sucrose, Sorbitol and Ethanol

Processes

Ke Tao¹  Jing Huang¹  Qiurong Qing¹  Shixian Zhao¹  Jincan Lei¹  Qiong Wang¹  Ke Sun¹  Ning Hu²  Jun Yang² 

[1] Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing 401331, China;Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China;

关键词: giant lipid vesicle;    glucose;    sucrose;    sorbitol;    ethanol;   

DOI  :  10.3390/pr9060945

来源: DOAJ

【 摘 要 】

Lipid vesicles, especially giant lipid vesicles (GLVs), are usually adopted as cell membrane models and their preparation has been widely studied. However, the effects of some nonelectrolytes on GLV formation have not been specifically studied so far. In this paper, the effects of the nonelectrolytes, including sucrose, glucose, sorbitol and ethanol, and their coexistence with sodium chloride, on the lipid hydration and GLV formation were investigated. With the hydration method, it was found that the sucrose, glucose and sorbitol showed almost the same effect. Their presence in the medium enhanced the hydrodynamic force on the lipid membranes, promoting the GLV formation. GLV formation was also promoted by the presence of ethanol with ethanol volume fraction in the range of 0 to 20 percent, but higher ethanol content resulted in failure of GLV formation. However, the participation of sodium chloride in sugar solution and ethanol solution stabilized the lipid membranes, suppressing the GLV formation. In addition, the ethanol and the sodium chloride showed the completely opposite effects on lipid hydration. These results could provide some suggestions for the efficient preparation of GLVs.

【 授权许可】

Unknown