Phage Display Selection of an Anti-Idiotype-Antibody with Broad-Specificity to Deoxynivalenol Mycotoxins

Toxins

Markus Vehniäinen¹  Janne Leivo²  Urpo Lamminmäki² 

[1] Biovian Ltd., 20520 Turku, Finland;Department of Biochemistry, University of Turku, 20520 Turku, Finland;

关键词: antibody library;    phage display;    mycotoxin;    deoxynivalenol;    immunoassay;   

DOI  :  10.3390/toxins13010018

来源: DOAJ

【 摘 要 】

The use of synthetic antibody libraries and phage displays provides an efficient and robust method for the generation of antibodies against a wide range of targets with highly specific binding properties. As the in vitro selection conditions can be easily controlled, these methods enable the rapid generation of binders against difficult targets such as toxins and haptens. In this study, we used deoxynivalenol mycotoxin as a target to generate anti-idiotype-antibodies with unique binding properties from synthetic antibody libraries. The binding of the selected anti-idiotype antibodies can be efficiently inhibited with the addition of free isoforms of deoxynivalenol. The antibody was consecutively used to develop deoxynivalenol-specific ELISA and TRF-immunoassays, which can detect deoxynivalenol and two of the most common metabolic isoforms in the range of 78–115 ng/mL.

【 授权许可】

Unknown