Characterization of Alternaria Species Associated with Heart Rot of Pomegranate Fruit

Journal of Fungi

Ilenia Siciliano¹  MariaLodovica Gullino¹  SimonaMarianna Sanzani²  SantaOlga Cacciola³  Francesco Aloi³  Antonella Pane³  Mario Riolo³  Annamaria Mincuzzi⁴  Antonio Ippolito⁴ 

[1] Agroinnova—Centre of Competence for the Innovation in the Agro-Environmental Sector, University of Turin, 10095 Turin, Italy;CIHEAM Bari, Via Ceglie 9, 70010 Valenzano, Italy;Department of Agriculture, Food and Environment, University of Catania, 95123 Catania, Italy;Department of Soil, Plant, and Food Sciences, University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy;

关键词: Alternaria alternata;    Alternaria arborescens;    morphotypes;    four-gene phylogeny;    mycotoxins;   

DOI  :  10.3390/jof7030172

来源: DOAJ

【 摘 要 】

This study was aimed at identifying Alternaria species associated with heart rot disease of pomegranate fruit in southern Italy and characterizing their mycotoxigenic profile. A total of 42 Alternaria isolates were characterized. They were obtained from pomegranate fruits with symptoms of heart rot sampled in Apulia and Sicily and grouped into six distinct morphotypes based on macro- and microscopic features. According to multigene phylogenetic analysis, including internal transcribed spacer (ITS), translation elongation factor 1-α (EF-1α), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and a SCAR marker (OPA10-2), 38 isolates of morphotypes 1 to 5 were identified as Alternaria alternata, while isolates of morphotype 6, all from Sicily, clustered within the Alternaria arborescens species complex. In particular, isolates of morphotype 1, the most numerous, clustered with the ex-type isolate of A. alternata, proving to belong to A. alternata. No difference in pathogenicity on pomegranate fruits was found between isolates of A. alternata and A. arborescens and among A. alternata isolates of different morphotypes. The toxigenic profile of isolates varied greatly: in vitro, all 42 isolates produced tenuazonic acid and most of them other mycotoxins, including alternariol, alternariol monomethyl ether, altenuene and tentoxin.

【 授权许可】

Unknown