Comprehensive investigation on the synergistic antibacterial activities of Jatropha curcas pressed cake and seed oil in combination with antibiotics

AMB Express

Haji Khan¹  Maleeha Siddiqi²  Alam Khan²  Safia Ahmed²  Malik Badshah²  Syeda Zakia Batool²  Fariha Hasan²  Aamer Ali Shah²  Abdul Haq²  Samiullah Khan²  Dildar Khan³  Arshad Islam⁴ 

[1] Centre for Biotechnology and Microbiology, University of Swat;Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University;Department of Pharmacy, Quaid-i-Azam University;Postgraduate Program in Physiology and Pharmacology, Institute of Biological Sciences, Universidade Federal de Minas Gerais;

关键词: Jatropha curcas;    Antibiotics;    Multidrug resistant bacteria;    Combinatorial therapy;    Fractional inhibitory concentration index;    Synergism;   

DOI  :  10.1186/s13568-019-0793-6

来源: DOAJ

【 摘 要 】

Abstract Synergistic combinations of various antimicrobial agents are considered ideal strategies in combating clinical and multidrug resistant (MDR) infections. In this study, antibacterial potential of Jatropha curcas crude seed extracts, seed oil, commercially available antibiotics, and their combinations were investigated for their synergistic effect against clinical, MDR and ATCC bacterial strains by agar well diffusion assay. Methanolic extracts remained more active against Staphylococcus aureus (ATCC), with zone of inhibition (ZOI) of 21 mm, than clinical and methicillin-resistant S. aureus (MRSA) strains (ZOI range ~ 15.0–17.0 mm). Molecular docking demonstrated that beta-monolaurin from methanolic extract exhibited greater affinity conformation for UDP-N-acetylmuramoyl-tripeptide–d-alanyl-d-alanine (MurF) ligase’s active pocket with binding energy of -7.3 kcal/mol. Moxifloxacin exhibited greater activity against Escherichia coli (ATCC) (ZOI ~ 50.0 mm), followed by ofloxacin against Pseudomonas chlororaphis (47.3 mm), moxifloxacin against P. monteilii (47 mm), P. aeruginosa (46.3 mm) and MRSA2 (46 mm) and ofloxacin against S. aureus (ATCC) strains (45.7 mm). Methanolic extract in combination with rifampicin showed the highest synergism against MRSA strains, A. baumannii, E. coli, E. faecalis, S. aureus, and P. aeruginosa, A. baumannii (MDR strain), P. chlororaphis, E. coli ATCC25922 and S. aureus ATCC25923. In combinations, moxifloxacin exhibited the highest antagonism. The methanolic, n-hexane, aqueous extracts and seed oil in various combinations with antibiotics showed 44.71, 32.94, 9.41 and 25.88% synergism, respectively. The current study showed that potency of antibiotics was improved when screened in combination with J. curcas seed’s components, supporting the drug combination strategy to combat antibacterial resistance.

【 授权许可】

Unknown