Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity

Cell Reports

Xiaoji Wu¹  Renchao Chen¹  Lan Jiang¹  Yi Zhang¹ 

[1] Howard Hughes Medical Institute, Boston Children’s Hospital, Boston, MA 02115, USA;

关键词: single-cell RNA-seq;    hypothalamus;    cell heterogeneity;    oligodendrocyte;    tanycyte;    neuron diversity;   

DOI  :  10.1016/j.celrep.2017.03.004

来源: DOAJ

【 摘 要 】

The hypothalamus is one of the most complex brain structures involved in homeostatic regulation. Defining cell composition and identifying cell-type-specific transcriptional features of the hypothalamus is essential for understanding its functions and related disorders. Here, we report single-cell RNA sequencing results of adult mouse hypothalamus, which defines 11 non-neuronal and 34 neuronal cell clusters with distinct transcriptional signatures. Analyses of cell-type-specific transcriptomes reveal gene expression dynamics underlying oligodendrocyte differentiation and tanycyte subtypes. Additionally, data analysis provides a comprehensive view of neuropeptide expression across hypothalamic neuronal subtypes and uncover Crabp1+ and Pax6+ neuronal populations in specific hypothalamic sub-regions. Furthermore, we found food deprivation exhibited differential transcriptional effects among the different neuronal subtypes, suggesting functional specification of various neuronal subtypes. Thus, the work provides a comprehensive transcriptional perspective of adult hypothalamus, which serves as a valuable resource for dissecting cell-type-specific functions of this complex brain region.

【 授权许可】

Unknown