Evolution of a plant gene cluster in Solanaceae and emergence of metabolic diversity

eLife

Shin-Han Shiu¹  Craig A Schenck²  Rachel Combs³  Pengxiang Fan³  Yann-Ru Lou³  Robert L Last⁴  Peipei Wang⁴  Federica Brandizzi⁴  Bryan J Leong⁴  Bethany M Moore⁴  Pengfei Cao⁵ 

[1] MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, United States;University of Wisconsin, Madison, United States;Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, United States;Department of Plant Biology, Michigan State University, East Lansing, United States;Division of Biological Sciences, University of Missouri, Columbus, United States;

关键词: Solanum lycopersicum;    Solanum quitoense;    Solanum pennellii;   

DOI  :  10.7554/eLife.56717

来源: DOAJ

【 摘 要 】

Plants produce phylogenetically and spatially restricted, as well as structurally diverse specialized metabolites via multistep metabolic pathways. Hallmarks of specialized metabolic evolution include enzymatic promiscuity and recruitment of primary metabolic enzymes and examples of genomic clustering of pathway genes. Solanaceae glandular trichomes produce defensive acylsugars, with sidechains that vary in length across the family. We describe a tomato gene cluster on chromosome 7 involved in medium chain acylsugar accumulation due to trichome specific acyl-CoA synthetase and enoyl-CoA hydratase genes. This cluster co-localizes with a tomato steroidal alkaloid gene cluster and is syntenic to a chromosome 12 region containing another acylsugar pathway gene. We reconstructed the evolutionary events leading to this gene cluster and found that its phylogenetic distribution correlates with medium chain acylsugar accumulation across the Solanaceae. This work reveals insights into the dynamics behind gene cluster evolution and cell-type specific metabolite diversity.

【 授权许可】

Unknown