Functional Neuronal Topography: A Statistical Approach to Micro Mapping Neuronal Location

Frontiers in Neural Circuits

Luke R. Johnson¹  Craig McDonald²  Alison Wright³  Hadley C. Bergstrom⁵  Anne Overell⁷  Angela Jacques⁷  Nicholas Chaaya⁷  Andrew R. Battle⁹ 

[1] Department of Psychiatry and Center for the Study of Traumatic Stress, Uniformed Services University School of Medicine, Bethesda, MD, United States;Department of Psychology, George Mason University, Fairfax, VA, United States;Faculty of Health Science and Medicine, Bond University, Gold Coast, QLD, Australia;Institute for Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, Australia;Psychological Science Department, Vassar College, Poughkeepsie, NY, United States;School of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD, Australia;School of Psychology and Counseling, Queensland University of Technology, Brisbane, QLD, Australia;Translational Research Institute, Brisbane, QLD, Australia;Translational Research Institute, School of Medicine, The University of Queensland Diamantina Institute, Brisbane, QLD, Australia;

关键词: microanatomy;    memory;    network;    allocation;    cluster;    topography;   

DOI  :  10.3389/fncir.2018.00084

来源: DOAJ

【 摘 要 】

In order to understand the relationship between neuronal organization and behavior, precise methods that identify and quantify functional cellular ensembles are required. This is especially true in the quest to understand the mechanisms of memory. Brain structures involved in memory formation and storage, as well as the molecular determinates of memory are well-known, however, the microanatomy of functional neuronal networks remain largely unidentified. We developed a novel approach to statistically map molecular markers in neuronal networks through quantitative topographic measurement. Brain nuclei and their subdivisions are well-defined – our approach allows for the identification of new functional micro-regions within established subdivisions. A set of analytic methods relevant for measurement of discrete neuronal data across a diverse range of brain subdivisions are presented. We provide a methodology for the measurement and quantitative comparison of functional micro-neural network activity based on immunohistochemical markers matched across individual brains using micro-binning and heat mapping within brain sub-nuclei. These techniques were applied to the measurement of different memory traces, allowing for greater understanding of the functional encoding within sub-nuclei and its behavior mediated change. These approaches can be used to understand other functional and behavioral questions, including sub-circuit organization, normal memory function and the complexities of pathology. Precise micro-mapping of functional neuronal topography provides essential data to decode network activity underlying behavior.

【 授权许可】

Unknown