A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices

Micromachines

Trieu Nguyen¹  Anders Wolff¹  Aaydha Chidambara Vinayaka²  Dang Duong Bang² 

[1] Department of Biotechnology and Biomedicine, Technical University of Denmark, DK 2800 Kgs. Lyngby, Denmark;Laboratory of Applied Micro and Nanotechnology (LAMINATE), Division of Microbiology and Production, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK 2800 Lyngby, Denmark;

关键词: polymer;    microfluidic chips;    point of care;    three-dimensional microstructures;    protocol;   

DOI  :  10.3390/mi10090624

来源: DOAJ

【 摘 要 】

This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of Salmonella (Salmonella Typhimurium and Salmonella Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR).

【 授权许可】

Unknown