Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat products

Brazilian Journal of Microbiology

Flávia Z. Freitas¹  Elaine C.P. De Martinis¹  Márcia R.P. Públio¹  Priscila R. Santarosa¹ 

[1] Universidade de São Paulo;

关键词: lactic acid bacteria;    meat;    bacteriocins;    biopreservation;    Listeria monocytogenes;   

DOI  :  10.1590/S1517-83822001000100008

来源: DOAJ

【 摘 要 】

Twenty samples of Brazilian meat and meat products were screened by the agar overlay method for bacteriocin-producing lactic acid bacteria, using Lactobacillus sake ATCC 15521 as indicator strain. Based on Gram staining, KOH reaction, catalase test and fermentation of 49 carbohydrates (API 50 CH), three out of seven isolates with confirmed antagonist properties were identified as Lactobacillus curvatus, one as Leuconostoc mesenteroides and one as Leuconostoc sp. Two isolates could not be properly identified using these tests. The inhibitors produced by these strains were sensitive to proteases. Inhibition due to lytic bacteriophages was ruled out, so the isolates were classified as bacteriocin-producing lactic acid bacteria. Four of them presented antilisterial activity and a potential application as biopreservatives in meat systems.

【 授权许可】

Unknown