Defining the role of pulmonary endothelial cell heterogeneity in the response to acute lung injury

eLife

Collin T Stabler¹  Apoorva Babu¹  Edward E Morrisey²  Jarod A Zepp²  John P Leach³  Su Zhou³  Michael P Morley⁴  Terren K Niethamer⁴ 

[1] Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, United States;Penn Cardiovascular Institute, University of Pennsylvania, Philadelphia, United States;Penn-Children's Hospital of Philadelphia Lung Biology Institute, University of Pennsylvania, Philadelphia, United States;Department of Medicine, University of Pennsylvania, Philadelphia, United States;

关键词: pulmonary endothelial cells;    vascular biology;    lung regeneration;    acute lung injury;   

DOI  :  10.7554/eLife.53072

来源: DOAJ

【 摘 要 】

Pulmonary endothelial cells (ECs) are an essential component of the gas exchange machinery of the lung alveolus. Despite this, the extent and function of lung EC heterogeneity remains incompletely understood. Using single-cell analytics, we identify multiple EC populations in the mouse lung, including macrovascular endothelium (maEC), microvascular endothelium (miECs), and a new population we have termed Car4-high ECs. Car4-high ECs express a unique gene signature, and ligand-receptor analysis indicates they are primed to receive reparative signals from alveolar type I cells. After acute lung injury, they are preferentially localized in regenerating regions of the alveolus. Influenza infection reveals the emergence of a population of highly proliferative ECs that likely arise from multiple miEC populations and contribute to alveolar revascularization after injury. These studies map EC heterogeneity in the adult lung and characterize the response of novel EC subpopulations required for tissue regeneration after acute lung injury.

【 授权许可】

Unknown