Performance of a Biodegradable Composite with Hydroxyapatite as a Scaffold in Pulp Tissue Repair

Polymers

Ayato Sugiyama¹  Ryuji Kato¹  Kei Kanie¹  Aika Yamawaki-Ogata²  Yuji Narita²  Shigetada Kawabata³  Yujiro Hirose³  Sayako Matsumoto⁴  Manahil Ali⁴  Yuki Ito⁴  Mikako Hayashi⁴  Masakatsu Watanabe⁴  Yusuke Takahashi⁴  Motoki Okamoto⁴  Hailing Huang⁴  Jiro Miura⁵  Koichiro Uto⁶  Mitsuhiro Ebara⁶ 

[1] Department of Basic Medicinal Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan;Department of Cardiac Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan;Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan;Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan;Division for Interdisciplinary Dentistry, Osaka University Dental Hospital, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan;International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1 Chome-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan;

关键词: pulp capping;    tertiary dentin;    polymer complex;    RNA sequence;   

DOI  :  10.3390/polym12040937

来源: DOAJ

【 摘 要 】

Vital pulp therapy is an important endodontic treatment. Strategies using growth factors and biological molecules are effective in developing pulp capping materials based on wound healing by the dentin-pulp complex. Our group developed biodegradable viscoelastic polymer materials for tissue-engineered medical devices. The polymer contents help overcome the poor fracture toughness of hydroxyapatite (HAp)-facilitated osteogenic differentiation of pulp cells. However, the composition of this novel polymer remained unclear. This study evaluated a novel polymer composite, P(CL-co-DLLA) and HAp, as a direct pulp capping carrier for biological molecules. The biocompatibility of the novel polymer composite was evaluated by determining the cytotoxicity and proliferation of human dental stem cells in vitro. The novel polymer composite with BMP-2, which reportedly induced tertiary dentin, was tested as a direct pulp capping material in a rat model. Cytotoxicity and proliferation assays revealed that the biocompatibility of the novel polymer composite was similar to that of the control. The novel polymer composite with BMP-2-induced tertiary dentin, similar to hydraulic calcium-silicate cement, in the direct pulp capping model. The BMP-2 composite upregulated wound healing-related gene expression compared to the novel polymer composite alone. Therefore, we suggest that novel polymer composites could be effective carriers for pulp capping.

【 授权许可】

Unknown