One-Step Surface Immobilization of Protein A on Hydrogel Nanofibers by Core-Shell Electrospinning for Capturing Antibodies

International Journal of Molecular Sciences

Kosuke Moriyama¹  Shin-ichiro Suye¹  Satoshi Fujita¹  Chihiro Naganuma¹ 

[1] Department of Frontier Fiber Technology and Science, University of Fukui, Fukui 910-8507, Japan;

关键词: surface modification;    protein A;    electrospinning;    nanofiber;    membrane chromatography;   

DOI  :  10.3390/ijms22189857

来源: DOAJ

【 摘 要 】

Nanofibers (NFs) are potential candidates as filter materials for affinity separation owing to their high liquid permeability based on their high porosity. Multiple and complex processes were conventionally performed to immobilize proteins for modifying NF surfaces. A simple method must be developed to immobilize proteins without impairing their biological activity. Herein, we succeeded in fabricating NFs with a core of cellulose acetate and a shell of hydrophilic polyvinyl alcohol immobilized with staphylococcal recombinant protein A by a one-step process based on core-shell electrospinning. A total of 12.9 mg/cm³ of antibody was captured in the fiber shell through high affinity with protein A immobilized in an aqueous environment of the hydrogel. The maximum adsorption site and dissociation constant evaluated by the Langmuir model were 87.8 µg and 1.37 µmol/L, respectively. The fiber sheet withstood triplicate use. Thus, our NF exhibited high potential as a material for membrane chromatography.

【 授权许可】

Unknown