Effect of Cryopreservation Method Supported with Biochemical Analyses in the Axillary Bud of Jewel Orchid, Ludisia discolor

Plants

Vanitha Mariappan¹  Suganthi Appalasamy²  Ranjetta Poobathy³  Bee Lynn Chew⁴  Kirutika Selva Rajan⁴  Sreeramanan Subramaniam⁴  Hazirah Burkhan⁴ 

[1] Centre of Toxicology and Health Risk Studies (CORE), Faculty of Health Sciences, Universiti Kebangsaan Malaysia (UKM), Kuala Lumpur 50300, Federal Territory of Kuala Lumpur, Malaysia;Department of Natural Resource and Sustainability, Faculty of Earth Science, Universiti Malaysia Kelantan (UMK), Locked Bag No. 100, Jeli 17600, Kelantan, Malaysia;School of Biological Sciences, Quest International University (QUIP), Ipoh 30250, Perak, Malaysia;School of Biological Sciences, Universiti Sains Malaysia (USM), Georgetown 11800, Penang, Malaysia;

关键词: axillary bud;    cryopreservation;    droplet-vitrification;    Ludisia discolor;    orchid;    reactive oxygen species;   

DOI  :  10.3390/plants11070879

来源: DOAJ

【 摘 要 】

This study investigated conserving an endangered terrestrial jewel orchid Ludisia discolor, using in vitro grown axillary buds. Excised segments of axillary buds (4–5 mm in length) were precultured on a modified Murashige and Skoog (MS) medium supplemented with 0.2 M sucrose for 24 h and osmoprotected in a loading solution for 20 min. Then, axillary buds were dehydrated in plant vitrification solution 2 (PVS2) for 10 min at 0 °C and incubated in liquid nitrogen for 1 h. Subsequently, axillary buds were rewarmed rapidly by dilution solution and transferred to a growth recovery medium supplemented with 0.05 µM melatonin, which led to an improved survival chance (16.67%) for cryopreserved L. discolor. The osmotic stress and the overproduction of reactive oxygen species (ROS) during cryopreservation stages may result in cryoinjuries and poor survival as increased levels of proline (5.51 µmol/g), catalase (85.64 U/g), peroxidase (565.37 U/g), and ascorbate peroxidase activities (12.19 U/g) were detected after dehydration, preculture, rewarming, and loading stage, respectively. Results obtained from this study indicate that further experimental designs which apply different PVS and exogenous antioxidants are needed for improved survival and regrowth of L. discolor.

【 授权许可】

Unknown