Biogeography of the Respiratory Tract Microbiome in Patients With Malignant Tracheal Tumors

Frontiers in Oncology

Hai-Xia Liu¹  Mei-Mei Tao²  Nan Zhang²  Yun-Zhi Zhou³  Hong-Wu Wang⁴  Kai-Xiong Liu⁶  Jing Zhang⁷  Jie-Ming Qu⁹ 

[1] Department of Infectious Disease, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Oncology, Emergency General Hospital, Beijing, China;Department of Respiratory Medicine, Emergency General Hospital, Beijing, China;Department of Respiratory and Critical Care Medicine, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, China;Department of Respiratory and Critical Care Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China;Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China;Department of Respiratory and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China;Institute of Respiratory Diseases, School of Medicine, Shanghai Jiao Tong University, Shanghai, China;Shanghai Key Laboratory of Emergency Prevention Diagnosis and Treatment of Respiratory Infectious Disease, Shanghai, China;

关键词: malignant tracheal tumor;    squamous cell carcinoma;    salivary gland type carcinoma;    16S rRNA sequencing;    microbiome;   

DOI  :  10.3389/fonc.2021.758917

来源: DOAJ

【 摘 要 】

BackgroundThis study aimed to characterize the bacterial microbiota in the oral cavity (OC), throat, trachea, and distal alveoli of patients with primary malignant tracheal tumors (PMTT), including squamous cell carcinoma (SCC) and salivary gland carcinoma patients (SGC), for comparison with a matched non-malignant tracheal tumor (NMTT) group.MethodsPatients with pathological diagnosis of PMTT and NMTT were included in this study. Saliva, throat swab (TS), trachea protected specimen brush (PSB), and bronchoalveolar lavage fluid (BALF) samples were collected for 16S rRNA gene sequencing. The composition, diversity, and distribution of the microbiota were compared among biogeographic sampling sites and patient groups. The relationship between the genera-level taxon abundance and tracheal tumor types was also investigated to screen for candidate biomarkers.FindingsThe most represented phyla in the four sites were Bacteroidetes, Firmicutes, Proteobacteria, and Fusobacteria. In SCC patients, the relative abundance of Bacteroidetes and Firmicutes gradually decreased with increasing depth into the respiratory tract, while the relative abundance of Proteobacteria gradually increased. Bacterial communities at the four biogeographic sites formed two distinct clusters, with OC and TS samples comprising one cluster and PSB and BALF samples comprising the other group. Principal coordinate analysis showed that trachea microbiota in SCC patients were distinct from that of SGC or NMTT patients. In the trachea, AUCs generated by Prevotella and Alloprevotella showed that the abundance of these genera could distinguish SCC patients from both NMTT and SGC patients.InterpretationThe structure of respiratory tract microbiota in PMTT patients is related to tumor type. Certain bacteria could potentially serve as markers of SCC, although verification with large-sample studies is necessary.

【 授权许可】

Unknown