Dietary supplementation of soybean-derived sterols regulates cholesterol metabolism and intestinal microbiota in hamsters

Journal of Functional Foods

Zesheng Zhang¹  Cuiru Diao²  Yong Yan²  Dong Liu²  Xiang Li²  Hao Wang²  Jing Cheng²  Fuping Zheng² 

[1] School of Marine and Biological Engineering, Yancheng Teachers University (YCTU), Yancheng, China;State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, China;

关键词: Soybean-derived sterols;    High-fat diet;    Cholesterol metabolism;    Sterol excretion;    Gut microbiota;   

DOI  :  

来源: DOAJ

【 摘 要 】

This study explored the of plant sterol-modulated cholesterol metabolism. Hamsters (n = 45) were divided into the control group, high-fat diet (HFD) group, and three treatment groups fed HFD supplemented with various soybean sterols (SS; 0.1%, 0.5% and 1%) concentrations; they were weighed weekly and sacrificed at 6 weeks. Serum lipids and liver cholesterol were determined. Fecal sterol amounts were assessed (gas chromatography/mass spectrometry). The liver and intestine mRNA levels of cholesterol absorption/sterol excretion associated genes (SREBP2, HMG-CoA-R, CYP7A1, LDL-R, NPC1L1, ABCG5, ABCG8, ACAT2, and MTP) were assessed by qRT-PCR. Intestinal microbiota was characterized by gene sequencing. Fecal amounts of neutral/acid sterols were increased by SS upon HFD feeding. Consistently, dietary SS generally upregulated cholesterol absorption/sterol excretion associated genes, including LDL-R, NPC1L1, ABCG5 and ABCG8. Furthermore, SS noticeably altered the gut microbiota. Overall, dietary SS as some impact on cholesterol metabolism and sterol excretion, possibly by altering specific genes and the gut microbiota.

【 授权许可】

Unknown