Characterization of Sodium Channel Mutations in the Dengue Vector Mosquitoes Aedes aegypti and Aedes albopictus within the Context of Ongoing Wolbachia Releases in Kuala Lumpur, Malaysia

Insects

StevenP. Sinkins¹  NoorAfizah Ahmad²  MuhammadKamarul Ridhuan Ghazali²  YoonLing Cheong²  NurZatil Aqmar Mohd Zabari²  NurRuqqayah Mohd Mazni²  SitiNor Syazwani Amran²  MohdArif Abdul Karim²  NazniWasi Ahmad²  AryA. Hoffmann³  NancyM. Endersby-Harshman³ 

[1] Institute of Infection, Immunity and Inflammation, MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, UK;Medical Entomology Unit, Institute for Medical Research, Ministry of Health, Jalan Pahang, Kuala Lumpur 50588, Malaysia;PEARG, School of BioSciences, Bio21 Institute, The University of Melbourne, 30 Flemington Rd, Parkville, Victoria 3010, Australia;

关键词: pyrethroid resistance;    target site;    kdr;   

DOI  :  10.3390/insects11080529

来源: DOAJ

【 摘 要 】

Specific sodium channel gene mutations confer target site resistance to pyrethroid insecticides in mosquitoes and other insects. In Aedes mosquito species, multiple mutations that contribute to resistance vary in their importance around the world. Here, we characterize voltage sensitive sodium channel (Vssc) mutations in populations of Aedesaegypti from Kuala Lumpur, Malaysia, and look at their persistence in populations affected by ongoing Wolbachia releases (a dengue control measure). We also describe a Vssc mutation in Aedesalbopictus (F1534L) found for the first time in Malaysia. We show that there are three predominant Vssc haplotypes in Aedesaegypti in this region, which all persist with regular backcrossing, thereby maintaining the original genetic composition of the populations. We identify changes in genotype frequency in closed populations of Ae. aegypti maintained for multiple generations in laboratory culture, suggesting different fitness costs associated with the genotypes, some of which may be associated with the sex of the mosquito. Following population replacement of Ae. aegypti by Wolbachia in the target area, however, we find that the Vssc mutations have persisted at pre-release levels. Mosquitoes in two genotype classes demonstrate a type I pyrethroid resistance advantage over wildtype mosquitoes when exposed to 0.25% permethrin. This resistance advantage is even more pronounced with a type II pyrethroid, deltamethrin (0.03%). The results point to the importance of these mutations in pyrethroid resistance in mosquito populations and the need for regular backcrossing with male mosquitoes from the field to maintain similarity of genetic background and population integrity during Wolbachia releases.

【 授权许可】

Unknown