A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey

Molecules

IgorM. Kenyo¹  AnnaI. Repetskaya²  MikhailV. Gorlov³  IrynaI. Fomochkina⁴  AlinaA. Kulanova⁴  KaterynaV. Laikova⁴  AnatolyV. Kubyshkin⁴  Karim Deri⁴  MikhailV. Subbotkin⁴  IgorN. Kasich⁵  EleonoraE. Seidosmanova⁶  RefatZ. Useinov⁶  ViktoriyaV. Bekirova⁶  AlekseiS. Zaitsev⁶  SergeyA. Nazarov⁶  NatalyaN. Smagliy⁶  MaksymN. Shumskykh⁶  VolodymyrV. Oberemok⁶  AlisaM. Krasnodubets⁶  AnnaO. Meshcheryakova⁶  NikitaV. Gal’chinsky⁶  KseniaI. Dydik⁶  EdieL. Chelengerova⁶ 

[1] Academy of Bioresources and Environmental Management of V.I. Vernadsky Crimean Federal University, 95492 Agrarnoye, Crimea;Botanical Garden named after N.V. Bagrov, V.I. Vernadsky Crimean Federal University, Vernadsky Avenue 4, 29500 Simferopol, Crimea;D. Mendeleev University of Chemical Technology of Russia, Miusskaya sq. 9, 125047 Moscow, Russia;Medical Academy named after S.I. Georgievsky, V.I. Vernadsky Crimean Federal University, Lenin Avenue 5/7, 295051 Simferopol, Crimea;Rostov State Medical University, Nakhchivan Lane 29, 344022 Rostov-on-Don, Russia;Taurida Academy, V.I. Vernadsky Crimean Federal University, Vernadsky Avenue 4, 295007 Simferopol, Crimea;

关键词: antisense oligonucleotides;    antisense therapy;    DNA insecticides;    RNAi;    medicine;    agriculture;    forestry;   

DOI  :  10.3390/molecules23061302

来源: DOAJ

【 摘 要 】

Antisense oligonucleotides (ASO), short single-stranded polymers based on DNA or RNA chemistries and synthesized in vitro, regulate gene expression by binding in a sequence-specific manner to an RNA target. The functional activity and selectivity in the action of ASOs largely depends on the combination of nitrogenous bases in a target sequence. This simple and natural property of nucleic acids provides an attractive route by which scientists can create different ASO-based techniques. Over the last 50 years, planned and realized applications in the field of antisense and nucleic acid nanotechnologies have produced astonishing results and posed new challenges for further developments, exemplifying the essence of the post-genomic era. Today the majority of ASOs are chemically modified and/or incorporated within nanoparticles to enhance their stability and cellular uptake. This review critically analyzes some successful cases using the antisense approach in medicine to address severe diseases, such as Duchenne muscular dystrophy and spinal muscular atrophy, and suggests some prospective directions for future research. We also examine in detail the elaboration of unmodified insect-specific DNA insecticides and RNA preparations in the areas of agriculture and forestry, a relatively new branch of ASO that allows circumvention of the use of non-selective chemical insecticides. When considering the variety of successful ASO modifications with an efficient signal-to-noise ratio of action, coupled with the affordability of in vitro oligonucleotide synthesis and post-synthesis procedures, we predict that the next half-century will produce a fruitful yield of tools created from effective ASO-based end products.

【 授权许可】

Unknown