Post-ruminal effects of rumen-protected methionine supplementation with low protein diet using long-term simulation and in vitro digestibility technique

AMB Express

Junhu Yao¹  Imtiaz Hussain Raja Abbasi¹  Yangchun Cao¹  Ayman A. Swelum²  Mohamed E. Abd El-Hack³  Farzana Abbasi⁴ 

[1] College of Animal Science and Technology, Northwest A&F University;Department of Animal Production, College of Food and Agriculture Sciences, King Saud University;Department of Poultry, Faculty of Agriculture, Zagazig University;School of Life Science and Engineering, Southwest University of Science and Technology;

关键词: In-vitro digestibility;    Methionine;    Protein;    Post-ruminal;    Rusitec;   

DOI  :  10.1186/s13568-018-0566-7

来源: DOAJ

【 摘 要 】

Abstract Microbial degradation in the rumen and dietary availability of methionine amino acid have been reported as limiting in dairy ruminants. The aim of the present study was to examine the post-ruminal effects of feeding ruminants different concentrations of rumen-protected methionine (RPM) in low crude protein diets using the long-term rumen simulation method (Rusitec) followed by in vitro abomasum and ileum digestibility technique. The experiment contained four treatment groups: (1) high protein, without RPM supplementation (HP); (2) low protein, without RPM supplementation (LP); (3) low protein supplementation with low RPM (LPLM); and (4) low protein supplementation with high RPM (LPHM) mixed per 20 ± 0.04 g basal diet in every fermenter. The results showed that the LPLM and LPHM groups had significantly higher disappearance of crude protein and neutral detergent fiber in the abomasum and ileum than the HP treatment (P < 0.05) and were the same as the LP group (P > 0.05). The proportions of short-chain fatty acids and total volatile fatty acids in the abomasum and ileum were the same between the LPHM and HP groups (P > 0.05); however, the LPLM group was found to be significantly (P < 0.05) lower than the HP group and similar to the LP group (P > 0.05). Rusitec pH before or after changing feed bags and daily ammonia nitrogen production in the abomasum and ileum were non significantly (P > 0.05) different among all groups. In conclusion, RPM supplementation with low crude protein diets promoted post-ruminal digestibility and production of volatile fatty acids.

【 授权许可】

Unknown