Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction

Biomedicine & Pharmacotherapy

Mingzhi Liao¹  Yinuo Wang²  Ziyu Chen²  Dawood Warraich Ahmad²  Yaqi Zhou²  Yu Ni²  Jianhong Ren²  Changjian Du²  Ning Zhang²  Junyao Hou²  Yayu Li²  Deyu Long²  Luyu Yang²  Abdur Rehman²  Yingcui Yu³ 

[1] Corresponding authors.;College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China;College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China;

关键词: Bisphenol A(BPA);    Microenvironment;    Secretory proteins;    Single-cell transcriptome;    Cell-cell communication network;    Reproductive toxicology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Testicular development during juvenile is crucial for subsequent male reproductive function. However, it remains poorly understood about the contribution of the testis microenvironment to human germ cell maturation. Therefore, we systematically analyzed scRNA-seq transcriptome and found the dramatic changes in cell-type composition in human testis during puberty. Then we constructed cell-cell communication networks between germ cells and somatic cells in the juvenile testis, which may be achieved via immune-related pathways. Our results showed that maturation-promoting factors are the switches of the Sertoli cells that drive sperm maturation. Furthermore, we found that Bisphenol A(BPA) enhanced the maturation and growth of germ cells through the Sertoli cell's secretory protein. Finally, our results indicate Bisphenol A would lead to the dysregulation of secreted protein expression in Sertoli cells during spermatogenesis, which in turn has direct cytotoxicity to Sertoli cells. Bisphenol A is one of the underlying causes of non-obstructive azoospermia (NOA). In summary, our results reveal the reproductive toxicity and molecular mechanism of Bisphenol A in Sertoli cells and male reproduction. Provide a reference for the toxicity of Bisphenol A to human reproduction.

【 授权许可】

Unknown