Dehydroabietic Acid Suppresses Inflammatory Response Via Suppression of Src-, Syk-, and TAK1-Mediated Pathways

International Journal of Molecular Sciences

Yong-Jin Kim¹  Donghyun Kim¹  Minkyeong Jo¹  Young-Gyu Kang²  Jong-Hoon Kim²  ByongChul Yoo²  TaeRyong Lee³  JiHye Kim³  JaeYoul Cho³  Eunji Kim⁴ 

[1] Innovation Division, R&;Basic Research &D Center, AmorePacific Corporation, Yongin 17074, Korea;Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea;

关键词: dehydroabietic acid (DAA);    inflammation;    NF-κB;    AP-1;   

DOI  :  10.3390/ijms20071593

来源: DOAJ

【 摘 要 】

Dehydroabietic acid (DAA) is a naturally occurring diterpene resin acid derived from coniferous plants such as Pinus and Picea. Various bioactive effects of DAA have been studied including antibacterial, antifungal, and anticancer activities. However, the anti-inflammatory mechanism of DAA remains unclear. We evaluated the anti-inflammatory effect of DAA in macrophage cell lines. Dehydroabietic acid clearly reduced nitric oxide (NO) production and inflammatory gene expression decreased according to RT-PCR results. Dehydroabietic acid displayed anti-inflammatory activity at the transcriptional level in results from NF-κB- or AP-1-mediated luciferase assays. To identify the DAA target protein, we investigated NF-κB and AP-1 pathways by Western blotting analysis. Dehydroabietic acid suppressed the activity of proto-oncogene tyrosine protein kinase (Src) and spleen tyrosine kinase (Syk) in the NF-κB cascade and transforming growth factor beta-activated kinase 1 (TAK1) in the AP-1 cascade. Using overexpression strategies, we confirmed that DAA targeted these kinases. Our findings demonstrate the anti-inflammatory effects and molecular mechanism of DAA. This suggests that DAA has potential as a drug or supplement to ameliorate inflammation.

【 授权许可】

Unknown