Impacts of Elevated CO2 Levels on the Soil Bacterial Community in a Natural CO2-Enhanced Oil Recovery Area

Diversity

Fu Chen¹  Jing Ma¹  Run Chen²  Qianlin Zhu²  Shaoliang Zhang³  Zhanbin Luo³ 

[1] Jiangsu Key Laboratory of Coal-based Greenhouse Gas Control and Utilization, China University of Mining and Technology, Xuzhou 221008, China;Low Carbon Energy Institute, China University of Mining and Technology, Xuzhou 221008, China;School of Environmental Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221008, China;

关键词: CO₂-enhanced oil recovery;    CO₂ leakage;    soil microbial community;    microbial interactions;    network analysis;   

DOI  :  10.3390/d11050077

来源: DOAJ

【 摘 要 】

Knowledge of the interactions among different microorganisms is important to understand how ecological function transformation is affected by elevated CO₂ levels in CO₂-enhanced oil recovery (CO₂-EOR) sites. Molecular ecological networks were established to reveal the interactions among different microbes of the soil bacterial community with the high-throughput sequencing data of 16S rRNA genes. The results showed that these networks are a powerful tool to identify and explain the interactions and keystone species in the communities under elevated CO₂ pressure. The structures of networks under different CO₂ leakage concentrations were different as a result of the networks’ topology properties, such as node numbers, topological roles of individual nodes, and network hubs. These indicators imply that the interactions among different groups were obviously changed. Moreover, changes in the network structure were significantly correlated with soil pH value, which might suggest that the large CO₂ leakage affected the soil ecosystem functions by changing the network interactions. Additionally, the key microbial populations such as Bacteroidetes and Proteobacteria were distinguished based on network topology to reveal community structure and ecosystem functioning. The work developed in this study could help microbiologists to address some research questions that could not be approached previously, and, hence, might represent a new area of research for microbial ecology.

【 授权许可】

Unknown