Honey Bee Proteolytic System and Behavior Parameters under the Influence of an Electric Field at 50 Hz and Variable Intensities for a Long Exposure Time

Animals

Agnieszka Murawska¹  Adam Roman¹  Paweł Migdał¹  Aneta Strachecka²  Paweł Bieńkowski³ 

[1] Department of Environment, Hygiene and Animal Welfare, Wroclaw University of Environmental and Life Sciences, 25 C.K. Norwida St., 51-630 Wroclaw, Poland;Institute of Biological Basis of Animal Production, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland;Telecommunications and Teleinformatics Department, Wroclaw University of Science and Technology, 27 Wybrzeze Wyspianskiego St., 50-370 Wroclaw, Poland;

关键词: proteases;    behavior;    proteolytic enzymes;    electromagnetic field;    honey bee immunity;   

DOI  :  10.3390/ani11030863

来源: DOAJ

【 摘 要 】

The effect of an artificial electromagnetic field on organisms is a subject of extensive public debate and growing numbers of studies. Our study aimed to show the effect of an electromagnetic field at 50 Hz and variable intensities on honey bee proteolytic systems and behavior parameters after 12 h of exposure. Newly emerged worker bees were put into cages and exposed to a 50 Hz E-field with an intensity of 5.0 kV/m, 11.5 kV/m, 23.0 kV/m, or 34.5 kV/m. After 12 h of exposure, hemolymph samples were taken for protease analysis, and the bees were recorded for behavioral analysis. Six behaviors were chosen for observation: walking, flying, self-grooming, contact between individuals, stillness, and wing movement. Bees in the control group demonstrated the highest number of all behavior occurrences, except flying, and had the lowest protease activity. Bees in the experimental groups showed a lower number of occurrences of walking, self-grooming, and contacts between individuals than the control bees and had significantly higher protease activity than the control bees (except that of alkaline proteases in the 23.0 kV/m group).

【 授权许可】

Unknown