Entry Inhibition of Influenza Viruses with High Mannose Binding Lectin ESA-2 from the Red Alga Eucheuma serra through the Recognition of Viral Hemagglutinin

Marine Drugs

Akira Nishizono¹  Takemasa Sakaguchi²  Yuichiro Sato³  Takanori Kubo³  Kinjiro Morimoto³  Makoto Hirayama⁴  Kanji Hori⁴ 

[1] Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka,Hasama-machi, Yufu, Oita 879-5593, Japan;Department of Virology, Institute of Biomedical & Health Sciences, Hiroshima University,1-2-3 Kasumi, Minami-Ku, Hiroshima 734-8551, Japan;Faculty of Pharmacy, Yasuda Women's University, 6-13-1 Yasuhigashi, Asaminami-Ku,Hiroshima 731-0153, Japan;Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama,Higashi-Hiroshima 739-8528, Japan;

关键词: lectin;    red algae;    Eucheuma serra;    high mannose glycan;    anti-influenza activity;   

DOI  :  10.3390/md13063454

来源: DOAJ

【 摘 要 】

Lectin sensitivity of the recent pandemic influenza A virus (H1N1-2009) was screened for 12 lectins with various carbohydrate specificity by a neutral red dye uptake assay with MDCK cells. Among them, a high mannose (HM)-binding anti-HIV lectin, ESA-2 from the red alga Eucheuma serra, showed the highest inhibition against infection with an EC50 of 12.4 nM. Moreover, ESA-2 exhibited a wide range of antiviral spectrum against various influenza strains with EC50s of pico molar to low nanomolar levels. Besides ESA-2, HM-binding plant lectin ConA, fucose-binding lectins such as fungal AOL from Aspergillus oryzae and AAL from Aleuria aurantia were active against H1N1-2009, but the potency of inhibition was of less magnitude compared with ESA-2. Direct interaction between ESA-2 and a viral envelope glycoprotein, hemagglutinin (HA), was demonstrated by ELISA assay. This interaction was effectively suppressed by glycoproteins bearingHM-glycans, indicating that ESA-2 binds to the HA of influenza virus through HM-glycans. Upon treatment with ESA-2, no viral antigens were detected in the host cells, indicating that ESA-2 inhibited the initial steps of virus entry into the cells. ESA-2 would thus be useful as a novel microbicide to prevent penetration of viruses such as HIV and influenza viruses to the host cells.

【 授权许可】

Unknown