Transcriptomic and metabolomic insights into the variety of sperm storage in oviduct of egg layers

Poultry Science

Ge Yang¹  Xuewen Hu¹  Xiaoliu Zhou¹  Qianqian Zhao¹  Yingfeng Tao¹  Zhiwei Liu¹  Shaomei Li¹  Shijun Li¹  Jinyu Chu¹  Chunyan Mou¹  Xinting Zheng¹  Fengying Shi²  Baogui Zhou²  Xiaoran Wei²  Shijie Fan² 

[1] Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;Poultry lnstitute, Huadu Yukou Poultry Industry Co. Ltd., Beijing 101206, China;

关键词: transcriptome;    metabolome;    sperm storage;    uterovaginal junction;    chicken;   

DOI  :  

来源: DOAJ

【 摘 要 】

In birds, the sperm storage tubules (SST) are dispersed in uterovaginal junction (UVJ) and highly correlated with differential capacity of sperm storage (SS) in and among species with unspecified mechanisms. Here, the SS duration of 252 egg layer breeders was evaluated in 5 rounds with 3 phenotypic traits to screen high- and low-SS individuals, respectively, followed with transcriptome of UVJ tissues and metabolome of serum (high-SS vs. low-SS) to decipher the candidate genes and biochemical markers correlated with differential SS capacity. Histological characterization suggested slightly higher density of SST in UVJ (high-SS vs. low-SS). Transcriptome analyses identified 596 differentially expressed genes (336 upregulated vs. 260 downregulated), which were mainly enriched in gene ontology terms of homeostasis, steroid and lipid metabolism and hormone activity, and 12 significant pathways (P < 0.05) represented by calcium, steroid, and lipid metabolism. Immunohistochemical staining of GNAQ, ST6GAL1, ADFP, and PCNA showed similar distribution in UVJ tissues between 2 groups. Several candidates (HSD11B2, DIO2, AQP3, GNAQ, NANS, ST6GAL1) combined with 4 (11β-prostaglandin F2α, prostaglandin B1, 7α-hydroxytestosterone, and N-acetylneuraminic acid) of 40 differential metabolites enriched in serum metabolome were considered as regulators and biomarkers of SS duration in egg layer breeders. The integrated transcriptome and metabolome analyses of chicken breeder hens will provide novel insights for exploration and improvement of differential SS capacity in birds.

【 授权许可】

Unknown