The Anti-Depressive Effects of Hesperidin and the Relative Mechanisms Based on the NLRP3 Inflammatory Signaling Pathway

Frontiers in Pharmacology

Haizhao Liu¹  Beitian Jia¹  Yuhong Bian¹  Zhimin Gu¹  Min Cao¹  Yiyang Wang¹  Lulu Xie¹  Zhaiyi Zhang¹  Ruiwen Song² 

[1] School of Intergrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China;School of Management, Tianjin University of Traditional Chinese Medicine, Tianjin, China;

关键词: depression;    inflammation;    microglia;    NLRP3;    hesperidin;   

DOI  :  10.3389/fphar.2020.01251

来源: DOAJ

【 摘 要 】

There is increasing evidence showing that inflammation is associated with depression in humans. Hesperidin, a natural bioflavonoid, has performed excellent effects on depression. The aim of this research was to investigate the therapeutic effect of hesperidin on chronic unpredictable mild stress (CUMS)-induced rats. The sucrose preference test (SPT), forced swimming test (FST), and open field test (OFT) were performed to measure the depression-related symptoms. The enzyme-linked immunosorbent assay (ELISA) was used to determine the concentrations of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in the prefrontal cortex (PFC) of rats and cellular supernatant. PCR and Western blot were used to monitor the differences of NLRP3, caspase-1, ASC activation in the levels of genes and proteins in the PFC of rats and microglia. The activation of microglia was determined using immunofluorescence staining and flow cytometry assay. Our results show that hesperidin treatment significantly relieved depressive like behaviors in CUMS rats. In addition, hesperidin decreased the expression levels of IL-1β, IL-6, TNF-α, NLRP3, caspase-1, and ASC in the PFC and microglia. This study investigated that hesperidin treatment ameliorated CUMS-induced depression by suppressing microglia and inflammation.

【 授权许可】

Unknown