Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensis

Food Science & Nutrition

Mai Sugi¹  Fukutaro Mizuhashi¹  Sawako Kasamoto¹  Sa Pham²  Barbara Danielewska‐Nikiel²  Ashley Roberts²  Christina Sulaiman²  Nobuo Okado³ 

[1] Biosafety Research Center, Foods, Drugs, and Pesticides (currently BioSafety Research Center Inc.) Shizuoka Japan;Intertek Scientific and Regulatory Consultancy Mississauga ON Canada;Shin Nihon Chemical Co., Ltd Aichi Japan;

关键词: Arabinase;    Aspergillus tubingensis;    food safety;    genotoxicity;    subchronic toxicity;   

DOI  :  10.1002/fsn3.1329

来源: DOAJ

【 摘 要 】

Abstract Arabinase is an enzyme recognized for its ability to degrade arabinan, a plant cell wall constituent. It has been applied in the food industry most commonly for juice processing. One commercial source of arabinase is Aspergillus tubingensis (A. tubingensis), a black Aspergillus species. Given the intended use in food for human consumption, and noting its potential presence at trace levels in finished products, a series of safety studies including in vitro Ames and chromosome aberration assays, in vivo mammalian erythrocyte micronucleus and alkaline comet assays, and a 90‐day rat oral toxicity study were conducted. No test article‐related mutagenic activity was observed in the Ames assay. Although positive activity was observed in the chromosome aberration assay, this was not replicated in the in vivo genotoxicity assays including in preabsorptive cells. In the subchronic toxicity study, no test article‐related adverse effects were observed following oral administration of arabinase at doses of 15.3, 153, or 1,530 mg total organic solids (TOS)/kg body weight/day to Sprague Dawley rats. The no‐observed‐adverse‐effect level was considered to be the highest dose tested (1,530 mg TOS/kg body weight/day). The results of the genotoxicity studies and the subchronic toxicity study support the safe use of arabinase from A. tubingensis in food production.

【 授权许可】

Unknown