The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells

Molecules

Yan-Chun Liu¹  Zhao-Ying Liu¹  Li Lin¹ 

[1] College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;

关键词: Gelsemium elegans;    gelsemine;    koumine;    PC12 cells;   

DOI  :  10.3390/molecules24102004

来源: DOAJ

【 摘 要 】

Two optical isomers, +/− gelsemine (1, 2), together with one known compound were isolated from the whole plant of G. elegans. The structures of the separated constituents were elucidated on 1D and 2D (¹H-¹H COSY, HMBC, HSQC) NMR spectroscopy and high-resolution mass spectrometry (HRMS). The isolated alkaloids were tested in vitro for cytotoxic potential against PC12 cells by the MTT assay. As a result, (+) gelsemine (compound 1) exhibited cytotoxic activity against PC12 cells with an IC₅₀ value of 31.59 μM, while (−) gelsemine (compound 2) was not cytotoxic.

【 授权许可】

Unknown